来源:研究生院  返回:ca88     日期:2018/12/26 11:10:54   点击数:5010  

时间:2018-12-26 14:30


报告人:Roya Mortazavi


【创源大讲堂】Next Generation Sequencing Analysis: Impact of Bacteria in Snow and Frost Flowers on the Arctic Ecosystem 下一代测序分析:雪和霜花中的细菌对北极生态系统的影响



主 讲 人: Roya Mortazavi

邀 请 人: 袁艳平


Roya Mortazavi, Ph.D., P.Eng, Department of Chemistry McGill University, Montreal, Canada. She has focus on the chemistry/Atmospheric &Oceanic Sciences, purification of water from Bacteria in Emergency Situation, risk Assessment for Airborne Particulate Matter for many years. UP to now, she has published more than 20 papers in international peer reviewed journals and give more than 13 presentations in international conferences.


Five different snow types and frost flowers in Barrow, Alaska, USA were examined for the identification, microbial population and their freezing nucleation activity. In addition to the culturing and gene sequence-based identification approach, the state-of-the-art genomic next-generation sequencing (NGS) technique was used to examine the diversity of bacterial communities in Arctic samples. Known phyla were detected (11-18) with the majority of sequences (12.3-83.1%) belonging to one of the five major phyla: Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Cyanobacteria. The number of genera detected ranged from, 101-245. Morphology and ice-nucleating abilities of the identified taxa and melted snow were obtained using both high resolution electron microscopy and ice nucleation cold-plate. Freezing point temperatures for bacterial isolates ranged from -20.3 ± 1.5 ℃ to -15.7 ± 5.6 ℃, and for melted samples from -9.5 ± 1.0 ℃ to -18.4 ± 0.1 ℃. An isolate belonging to the genus Bacillus (96% similarity) had ice nucleation activity of -6.8 ± 0.2 ℃. Comparison with Montreal urban snow, revealed that a seemingly diverse community of bacteria exists in the Arctic with some possibly originating from distinct ecological environments transported by Asian/African dust storm.






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